dsred2 pbad  (Addgene inc)

Journal: Frontiers in Molecular Biosciences

Article Title: Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins

doi: 10.3389/fmolb.2021.633217

Figure Lengend Snippet: Absorption and fluorescence properties of RFPs.

Article Snippet: DsRed2 and mPlum were encoded on pBAD; DsRed2-pBAD was a gift from Michael Davidson (Addgene plasmid # 54608; http://n2t.net/addgene:54608 ; RRID:Addgene_54608), and mPlum-pBAD was a gift from Michael Davidson and Roger Tsien (Addgene plasmid # 54564; http://n2t.net/addgene:54564 ; RRID:Addgene_54564).

Techniques: Fluorescence

Journal: Frontiers in Molecular Biosciences

Article Title: Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins

doi: 10.3389/fmolb.2021.633217

Figure Lengend Snippet: Permanent dipole moment difference vector with its length |Δ μ | and its direction (angle δ ) relatively to x -axis, as well as other parameters involved in finding Δ μ .

Article Snippet: DsRed2 and mPlum were encoded on pBAD; DsRed2-pBAD was a gift from Michael Davidson (Addgene plasmid # 54608; http://n2t.net/addgene:54608 ; RRID:Addgene_54608), and mPlum-pBAD was a gift from Michael Davidson and Roger Tsien (Addgene plasmid # 54564; http://n2t.net/addgene:54564 ; RRID:Addgene_54564).

Techniques: Plasmid Preparation

Journal: Frontiers in Molecular Biosciences

Article Title: Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins

doi: 10.3389/fmolb.2021.633217

Figure Lengend Snippet: Graphical solution of (blue curves) and (red curve), to find the Δ μ x and Δ μ y components of Δ μ for DsRed2. The structure of the DsRed2 chromophore is displayed in the background, illustrating the molecular system of coordinates. Green dashed bi-directional arrows depict the direction of the oscillating transition dipole moment μ , calculated in , for the chromophore in vacuum (light green) and in DsRed protein (dark green). The dotted light green arrow corresponds to a direction of μ calculated in vacuum in . Purple dashed arrows correspond to the quantum mechanically calculated Δ μ vector in DsRed protein environment described by three different models ; see text. The purple solid arrow is the selected Δ μ solution of . It was selected based on the known Δ μ and μ vectors calculated in and our MD simulations of the electric field; see text. Green solid bi-directional arrows represent the two possible directions of the μ vector based on our measurement of γ and the selected direction of Δ μ .

Article Snippet: DsRed2 and mPlum were encoded on pBAD; DsRed2-pBAD was a gift from Michael Davidson (Addgene plasmid # 54608; http://n2t.net/addgene:54608 ; RRID:Addgene_54608), and mPlum-pBAD was a gift from Michael Davidson and Roger Tsien (Addgene plasmid # 54564; http://n2t.net/addgene:54564 ; RRID:Addgene_54564).

Techniques: Plasmid Preparation

Journal: Frontiers in Molecular Biosciences

Article Title: Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins

doi: 10.3389/fmolb.2021.633217

Figure Lengend Snippet: Components of Δ μ vector and corresponding electric fields for a series of RFPs.

Article Snippet: DsRed2 and mPlum were encoded on pBAD; DsRed2-pBAD was a gift from Michael Davidson (Addgene plasmid # 54608; http://n2t.net/addgene:54608 ; RRID:Addgene_54608), and mPlum-pBAD was a gift from Michael Davidson and Roger Tsien (Addgene plasmid # 54564; http://n2t.net/addgene:54564 ; RRID:Addgene_54564).

Techniques: Plasmid Preparation